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Phytosterol alterations responding to ROS inhibitors by GC-MS in Arabidopsis

Yuanyuan Wang, Tianlin Jin, Xue Wang and Dacheng Liang

Phytosterols, essential components of cellular membrane integrity, play an important role in plant growth, development, and responses to various types of stressors. Emerging studies have proposed that phytosterols are a result of adaptation to the aerobic environment during evolution. We reasoned that phytosterols could dramatically respond to changes in external reactive oxygen species (ROS) levels. The levels of five phytosterols were measured by gas chromatography-mass spectrometry (GC-MS) after Arabidopsis plants were treated with the ROS-altering drugs 2’-3’-dideoxycytidine (DDC), H2O2, Catalase (CAT), diphenyleneiodonium (DPI), and salicylhydroxamic acid (SHAM) and auxin transport inhibitors 2,3,5-triiodobenzoic acid (TIBA) and 1-N-naphthylphthalamic acid (NPA). We found that all redox-altering agents can dramatically reduce sterol levels, whereas CAT and NPA can partly increase them. Our results establish a link between redox balance and sterol level alteration.  

To Cite this article: Wang, Y., T. Jin, X. Wang and D. Liang. 2022. Phytosterol alterations responding to ROS inhibitors by GC-MS in Arabidopsis.Pak. J. Bot., 54(3): DOI:  

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