PJB-2021-1513
Optimization of fermentation parameters for enhanced APHE production from Streptomyces griseocarneus through submerged fermentation
Abida Rafique, Ali Nawaz and Hamid Mukhtar
Abstract
Increasing resistance of microbes against antimicrobial drugs keep scientists busy in exploring novel and potent antibiotics. The present study is concerned with the production of antitumor antibiotic from Streptomyces griseocarneus NRRL B1068 using submerged fermentation technique. The antibiotic activity was tested against three test microorganisms including A. niger, E. coli and B. subtilis by agar well diffusion method. The production of antibiotic was enhanced by optimizing culture and physical conditions. Different culture media were screened and M1 medium consisting of (g/L), potassium dihydrogen phosphate, 3.24; dipotassium hydrogen phosphate, 5.65; hydrated magnesium sulphate, 1.0; and 1 ml stock solution of salts (ferrous sulphate, 0.1; manganese chloride, 0.1; and zinc sulphate, 0.1) supplemented with 7.5% glucose and 2.0% lysine was found best for antibiotic synthesis. Optimum temperature, pH and incubation period for the production of antitumor antibiotic were found to be 30ºC, 7.2 and 7 days, respectively. Seven days old inoculum in a concentration of 8% (v/v) was determined best for antitumor antibiotic production by Streptomyces griseocarneus NRRL B1068
To Cite this article:
Rafique, A., A. Nawaz and H. Mukhtar. 2021. Optimization of fermentation parameters for enhanced APHE production from Streptomyces griseocarneus through submerged fermentation. Pak. J. Bot., 53(2): DOI: http://dx.doi.org/10.30848/PJB2021-2(28)
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