PJB-2023-1524
Analysis of key biological pathways for sugar accumulation during kernel development in different genotypes of sweet corn
Huilin Yu, Yuqun Wu, Yang Zhang and Yanbo Wang
Abstract
Sweet corn as a nutritious, grain and vegetable crop plays a key role in the structural adjustment of the plant industry. The analysis of metabolic pathways of sugar accumulation during sweet corn kernel development is important for sweet corn breeding and efficient development. In this study, we used super sweet corn (T) and non-sweet corn (NT) as screened materials to carry out transcriptome analysis involved phenotypic parameters and physiological characteristics under two key periods of kernel development. Results showed that the key stage for the formation of sugar accumulation differences between super T and NT was 12 days after filling (GP12) to 20 days after filling (GP20), the proportion of soluble sugars of T decreased significantly compared with NT during this stage, while the proportion of sucrose and glucose of T increased significantly compared with NT. In terms of phenotypes, analysis showed that there was no significant difference in sugar accumulation between the two genotypes of sweet corn seeds at the two filling stages of GP12 and GP20. Transcriptome analysis revealed that 3175 genes were up-regulated and 3189 genes were up-regulated in the T and NT genotypes of sweet corn at the GP12 filling stage, while 3966 genes were up-regulated and 2809 genes were up-regulated at the GP20 filling stage, and clustering analysis indicated that LOC100382789, Zm00001d027703, and LOC1003043343 were significantly different from others. Meanwhile, GO analysis showed that seven biological processes, including Cyanoamino acid metabolism and Alanine aspartate, were the key regulatory pathways for sugar accumulation in sweet corn at GP12 and GP20 stages, among which Linoleic acid metabolism was significantly expressed in the middle and late stages of filling. In addition, KEGG enrichment differential analysis revealed that the key gene metabolic pathways Circadian rhythm-plant and Fatty acid metabolism were the key metabolic pathways for metabolic regulation of sugar accumulation in sweet corn. The study also mapped the target genes involved in metabolic pathways found by the KEGG enrichment analysis at the BP level, of which revealed 15 key metabolic pathways that are likely to be intrinsic to the differences in sugar accumulation between the two genotypes sweet corn. Sweet corn of super sweet genotype (T) could complete sugar accumulation through three types of key gene regulation, seven metabolic pathways and 15 metabolic pathways during the key stages of 12 days after filling (GP12) to 20 days after filling (GP20).
To Cite this article:
Yu, H., Y. Wu, Y. Zhang and Y. Wang . 2023. Analysis of key biological pathways for sugar accumulation during kernel development in different genotypes of sweet corn. Pak. J. Bot., 55(3): DOI: http://dx.doi.org/10.30848/PJB2023-3(17)
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