PJB-2023-1536
Exogenous application of proline and glycine betaine mitigates nickel toxicity in mung bean plants by up-regulating growth, physiological and yield attributes
Khuram Shahzad, Aamir Ali, Abdul Ghani, Muhammad Nadeem, Tehseen Khalid, Sarfraz Nawaz, Muhammad Jamil and Tauseef Anwar
Abstract
Nickel (Ni) toxicity is a serious threat to plant yield, especially when present in the soil, and its amount is increasing with each passing day. This study addresses the mitigation of this toxicity using proline and glycine betaine. The effect of exogenous glycine betaine (50, 100 mM) and proline (30, 60 mM) application on the morpho-physiological and yield attributes of Mung bean [Vigna radiata (L.) Wilczek] under varying levels of nickel stress (0, 100, 125, and 150 mg Ni/kg of soil) were investigated in this study. Data for growth and yield parameters were collected after 30, 45, and 65 days, respectively. The results showed that under nickel stress, root length, shoot length, fresh weight, chlorophyll contents, gas exchange characteristics, and yield attributes were reduced compared to the control. Foliar applications of proline (30, 60 mM) and glycine betaine (50 mM) improved all growth parameters, gas exchange characteristics, leaf carotenoid content, and yield attributes. The root length, shoot length, and gas exchange characteristics all correlated positively and significantly. On the other hand, root length, chlorophyll, and photosynthetic rate had a negative correlation. In Ni-stressed plants, the application of 100 mM glycine betaine caused additional damage. The exogenous application of 30 mM proline appeared to be the most effective treatment for nickel toxicity in mung bean plants. The results show that nickel toxicity has a negative impact on mung bean growth and yield, whereas optimal doses of both osmolytes have a high potential to alleviate nickel toxicity. The growth of mung bean was enhanced by foliar sprays of proline and glycine betaine, which improved gas exchange and increased chlorophyll and carotenoid levels. In the future, the identified treatments can optimally be used to obtain a high yield after the resilience development
To Cite this article:
Shahzad, K., A. Ali, A. Ghani, M. Nadeem, T. Khalid, S. Nawaz, M. Jamil and T. Anwar. 2023. Exogenous application of proline and glycine betaine mitigates nickel toxicity in mung bean plants by up-regulating growth, physiological and yield attributes. Pak. J. Bot., 55(SI): DOI: http://dx.doi.org/10.30848/PJB2023-SI(3)
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