PJB-2023-168
Rapid identification and genetic relationship of local varieties of peruviana L.
Gong Yuqiao, Liu Chen, Zhang Xiangyu, Shiguo Qiang and Qiao Hongyu
Abstract
Plant variety and cultivar discovery is one of the most significant and tricky issue in agricultural arrangement. This study is for barcoding of 12 Physalis peruviana L., landraces in northeast of China with three specific primers (ITS2, matK, rbcL) and their combinations loci such as rbcL+matK, ITS2+rbcL, ITS2+matK, ITS2+rbcL+matK. Otherwise, 10 genetic difference of landraces was assess using 10 SSR markers. The phylogenetic trees of ITS2+rbcL and rbcL+matK landraces of Physalis peruviana L., in a separate clade demonstrating that the combinations loci ITS2+rbcL and rbcL+matK have sufficient efficiency for landraces discrimination of Physalis peruviana. In addition, Mantel check showed no important correlation between genetic space and geographical distance. AMOVA analysis showed that the main Physalis peruviana L., landraces variation of the collection existed in the within-population or cluster
To Cite this article:
Yuqiao, G., L. Chen, Z. Xiangyu, S. Qiang and Q. Hongyu. 2025. Rapid identification and genetic relationship of local varieties of peruviana L. Pak. J. Bot., 57(1): DOI: http://dx.doi.org/10.30848/PJB2025-1(24)
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