Paper Details

PJB-2026-1516

Micropropagation and phytochemical analysis of leaf and fruit extracts of wild Himalayan grape (Vitis jacquemontii)

Tour Jan, Muhammad Sohail Ahmad, Sidra Gul, Sahar Nasim, Sarwat Naz, Sarah Ali, Lubna Faraz, Shaukat Ali, Muhammad Wahab and Kashif Ur Rahman
Abstract

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Vitis jacquemontii is a wild grape, and indigenous people use it as a substitute for cultivated grapes. Human activities pose a significant threat to the plant's biodiversity. Plants are disappearing due to these activities. Plant tissue culture is recommended for protecting plants through In vitro conservation methods. This study aimed to develop an effective method for inducing callus and shoots and also to analyze the chemical constituents in the fruit and leaf extracts. The maximum callus induction response (57%) was achieved from nodal explant weighing (3.12 grams) on an MS medium containing naphthalene acetic acid (NAA 1.5 mg/L). The MS medium supplemented with 1.5 mg/L 6-benzylaminopurine (BA) produced a maximum shoot proliferation of 8.34 per explant.  The highest rooting percentage (51.23%) and largest number of roots (5.62) of shoots were obtained on MS medium augmented with 1.0 mg/l NAA. HPLC analysis confirmed eight compounds in the Vitis jacquemontii fruit methanol extract (VJFME) and six in the leaf methanol extract (VJLME). Rutine was abundant in the VJLME extract, and quercetin was in the VJFME extract. Quantitative phytochemical analysis revealed higher total tannin (TTC), phenol (TPC), and flavonoids in the VJFME extract than in the VJLME extract. In conclusion, a regenerative system was established to produce materials from V. jacquemontii for bioactive compounds

To Cite this article: Jan, T., M.S. Ahmad, S. Gul, S. Nasim, S. Naz, S. Ali, L. Faraz, S. Ali, M. Wahab and K.U. Rahman. 2026. Micropropagation and phytochemical analysis of leaf and fruit extracts of wild Himalayan grape (Vitis jacquemontii). Pak. J. Bot., 58(5): DOI: http://dx.doi.org/10.30848/PJB2026-5(1)  
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