PJB-2013-217
BIOACTIVE ANALYSIS OF CHLOROPHYCOTA SPECIES VIA 96-WELL MICRO TITER PLATE TECHNIQUE
TASNEEM SAHER* AND ALIYA RAHMAN
Abstract
The 96- well Micro Titer Plate technique was conducted for various species of the phylum Chlorophycota against promastigote stage of Leishmania major. Among selected species of seaweeds Codium flabellatum (IC50 /72h = 34.4±6.2/µg/mL) Caulerpa faridii (IC50 /72h = 34.0±0.15/µg/mL) Caulerpa racemosa (IC50 /72h = 37.6±0.45/µg/mL) and Ulva fasciata (IC50 /72h = 50±0.65/µg/mL) displayed significant values, while the other tested species like Ulva lactuca IC50 /72h = 64.75±µg/m), Ulva rigida (IC50 /72 = 65.69±6.8/µg/m) and Codium iyangarri (IC50 /72h = 60.40µg/m) presented good activity. This result indicates that seaweeds of the phylum Chlorophycota possess antileishmanial activity which was conducted for the first time in Pakistan that could be utilized and employed beneficially.
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