PJB-2018-532
Influence of Trichoderma inoculation on four crucial defense-related enzymes and leaf soluble protein level of poplar
Yue-Feng Wang, Abdul Majeed Baloch, Jun-Jie Deng, Abdul Wahid Baloch, Xue-Yue Hou, Rui Miao and Rong-Shu Zhang
Abstract
Trichoderma spp. are beneficial endophytic plant symbionts known as plant growth improving factors and control agents for plant pathogens. We report in this study that the mechanisms for biological control of T. asperellum was the inducing of increases of crucial defense-related enzymes SOD, POD, PPO and PAL, and increasing the soluble protein level in leaf. Four T. asperellum strains each had slightly differential effects but all have positive effects. T. asperellum ACCC30536 had the best promoting effects. The activities of SOD and POD in poplar leaf responded to T. asperellum inducing as early as four hours after inoculation and both reached peak on the third day after inoculation. In contrast, the activities of both PPO and PAL in poplar leaf shortly showed suppression within one day after inoculation before the following drastic increase both peaking on the sixth day after inoculation. On the gene expression level, the regulation of SOD, POD and PAL in leaf showed consistent patterns with enzyme activities while PPO in leaf and the four enzymes in root indicated more complex regulatory mechanisms. Leaf soluble protein level were highly induced by each of the four T. asperellum strains and maintained almost stable from four hours after inoculation.
To Cite this article:
Wang, Y.F., A.M. Baloch, J.J. Deng, A.W. Baloch, X.Y. Hou, R. Miao and R.S. Zhang. 2020. Influence of Trichoderma inoculation on four crucial defense-related enzymes and leaf soluble protein level of poplar. Pak. J. Bot., 52(3): DOI: http://dx.doi.org/10.30848/PJB2020-3(11)
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