PJB-2018-712
Salt stress resistance in SmCP-transgenic Arabidopsis thaliana as revealed by transcriptome analysis
He Li, Liu Zheng, Ying Wang, Xianqi Hu, Zhuchou Lu, Huijin Fan, Zhuang Zhang, Yixiao Li, Renying Zhuo and Wenmin Qiu
Abstract
Adaptation to environmental changes is crucial for the viability of all organisms. In plants, cysteine proteases (CP) are vital proteolytic enzymes response to complex and volatile environmental factors. Previously, over-expression of a CP gene isolated from Salix matsudana (SmCP) was shown to improve the salt stress tolerance of Arabidopsis thaliana. However, the molecular mechanisms that underlie the enhanced salt stress tolerance of these over-expression lines remain uncharacterized. In this study, the transcriptome of transgenic Arabidopsis SmCP over-expression lines and wild type (WT) control (CT) plants was analyzed by RNA sequencing to identify genes associated with salt tolerance. The abundance level of selected differentially expressed genes was validated by quantitative real-time PCR analysis. The SmCP-transgenic line showed many transcriptomic changes under salt-stress conditions, including genes associated with alterations in the anti-oxidant environment and ion-transport capacity. Elucidation of the mechanism of salt stress resistance is important for utilization of SmCP for genetic improvement of commercial crops for tolerance to saline soil.
To Cite this article:
Li, H., L. Zheng, Y. Wang, X. Hu, Z. Lu, H. Fan, Z. Zhang, Y. Li, R. Zhuo and W. Qiu. 2020. Salt stress resistance in SmCP-transgenic Arabidopsis thaliana as revealed by transcriptome analysis. Pak. J. Bot., 52(3): DOI: http://dx.doi.org/10.30848/PJB2020-3(8)
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