PJB-2018-1021
Cadmium decreased superoxide anion derived from NADPH oxidase through overload of calcium in wheat seedling
Hongjuan Jing, Yi Fu, Cuixiang Li, Mengying Chen, Zewei Gu, Youtian Shan and Xiaorong Tan
Abstract
Cadmium (Cd) pollution is an important environmental problem in society. Cd is released by industrial processes and phosphate fertilizers, permeates into the food chain. It inhibits plant growth and changes photosynthesis, and its pytotoxicity damages the plant. Over-production of reactive oxygen species (ROS) is believed to be one of the key factors responsible for Cd toxicity. Our objective was to clarify the roles of ROS and cytoplasm free calcium ([Ca2+]cyt) in wheat seedlings containing cadmium (Cd). Wheat grains were treated by a series of doses of cadmium chloride for six days. Results showed that wheat grains germination and seedling growth inhibition were increased with increasing Cd concentration. Additionally, low Cd concentration (0.05 mM) decreased NADPH oxidase (NOX) activity, and superoxide anion (O2·-) and malondialdehyde (MDA) contents. Conversely, [Ca2+]cyt was significantly increased at 0.05 mM Cd. To obtain deeper insights into the redox balance of wheat seedling response to Cd stress, O2·-, H2O2 and MDA content In vivo were measured. The results showed that exogenous O2·markedly promoted seedling growth, increased O2·- content and NOX activity, and impaired [Ca2+]cyt overload by Cd stress. Therefore, it was concluded that Cd inhibited early growth of seedlings mainly through inducing [Ca2+]cyt overload which was attributed to the decrease of NOX-dependent O2·- production.
To Cite this article:
Jing, H., Y. Fu, C. Li, M. Chen, Z. Gu, Y. Shan and X. Tan. 2020. Cadmium decreased superoxide anion derived from NADPH oxidase through overload of calcium in wheat seedling. Pak. J. Bot., 52(5): DOI: http://dx.doi.org/10.30848/PJB2020-5(15)
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