PJB-2018-1050
Development of a dCAPS marker based on a minor resistance gene related to head smut in BIN8.03 in maize
Lin Zhang, Xuan Zhou, Xiaohui Sun, Yu Zhou, Xing Zeng, Zhoufei Wang, Zhenhua Wang and Hong Di
Abstract
Head smut can cause maize yield losses of up to 80% in the maize-growing region of Northern China. Molecular markers based on candidate resistance genes have proved to be useful for highly sensitive selection of resistance in maize breeding programs. In the present study, a SNP (single-nucleotide polymorphism) in resistant and susceptible maize lines was identified within the nucleotide-binding site (NBS)-leucine-rich repeat (LRR) gene GRMZM2G047152 in bin 8.03. Using this SNP, we developed the derived cleaved amplified polymorphic sequence marker DNdCAPS8.03-1. The molecular coincidence rate of the combination of markers DNdCAPS8.03-1 and LSdCAP2 (related to the QTL qHS2.09) was higher than that of a single marker in a set of 56 inbred maize lines belonging to five heterotic groups. These results provide information and tools that will prove to be useful in marker-assisted selection (MAS) in breeding maize for head smut resistance
To Cite this article:
Zhang, L., X. Zhou, X. Sun, Y. Zhou, X. Zeng, Z. Wang, Z. Wang and H. Di. 2020. Development of a dCAPS marker based on a minor resistance gene related to head smut in BIN8.03 in maize. Pak. J. Bot., 52(5): DOI: http://dx.doi.org/10.30848/PJB2020-5(17)
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