PJB-2019-364
Strain improvement by random mutagenesis of Aspergillus tamarii RMLC-10 for improved biosynthesis of polygalacturonase
Marium Munir, Roheena Abdullah, Ikram Ul Haq, Afshan Kaleem, Mehwish Iqtedar and Shagufta Naz
Abstract
Increasing and inevitable applications of polyagalacturonase in food and poultry feed industries urged the researchers to develop improved fungal strains for its industrial production. Polygalacturonase producing a wild strain of Aspergillus tamarii RMLC-10 was exposed to physical i.e. UV rays and chemical, i.e. Ethyl methanesulfonate (EMS), sodium azide and nitrous acid treatment to develop a mutant strain. This resulted in the development of forty-five variant strains. Among these, sodium azide treated a mutant strain of Aspergillus tamarii SA-11 showed a maximum increase in enzyme activity i.e. 32.01±0.09 U/ml compared to wild strain RMLC-10 with the enzyme activity of 24.57±0.04 U/ml. Effect of different cultural conditions such as incubation time, temperature and medium pH on polygalacturonase activity of wild and mutant strains was assessed. This showed an increase in enzyme activities of both wild (27.18±0.02 U/ml) and mutant (34.67±0.06) strains after an incubation time of 72 h at 30℃ in a fermentation medium of pH 6.
To Cite this article:
Munir, M., R. Abdullah, I.U. Haq, A. Kaleem, M. Iqtedar and S. Naz. 2020. Strain improvement by random mutagenesis of Aspergillus tamarii RMLC-10 for improved biosynthesis of polygalacturonase. Pak. J. Bot., 52(5): DOI: http://dx.doi.org/10.30848/PJB2020-5(29)
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