PJB-2022-1541
Establishment of an efficient cell suspension culture system for Lonicera japonica Thunb
Ning Wang, Hao Chen, Qinghai Gao and Jingjing Qian
Abstract
Lonicera japonica Thunb (L. japonica T.) has a high medicinal value. Establishing a cell suspension system lays the research foundation for large-scale extraction of the medicinal products of L. japonica T.. Using stem segments from L. japonica T. tissue culture seedling as material, the effects of various hormone combinations on the growth of L. japonica T. cells were studied, the effects of initial inoculum, pH and sucrose concentration of culture medium on cell suspension culture were studied, and the optimal culture time of cell suspension culture was screened. This experiment established an efficient cell suspension culture system from the L. japonica T., stem segment to provide a foundation for large-scale extraction. The following were the observed results: (1) Different hormone combinations had varying effects on the growth of L. japonica. 1.5 mg/L Indol-3-Butyric acid (IBA) had the best effect on plantlet rooting in japonica cells, while 1.0 mg/L 6-Benzylaminopurine (6-BA) was suitable for bud differentiation, and 0.2 mg/L 6-BA + 0.8 mg/L 1-Naphthaleneacetic acid (NAA) had the best effect on callus dedifferentiation. (2) For the optimal cell suspension culture system, the best initial inoculum weight was 50 g/L, with a pH of 5.8 and a sucrose concentration of 3%. (3) The cell growth curves were determined using the optimal cell suspension system described above. Cell growth peaked at 18 days of suspension culture, with the fresh weight of cells being 676.0±12.66 g/L; 13.52 times higher than the initial inoculation amount
To Cite this article:
Wang, N., H. Chen, Q. Gao and J. Qian. 2022. Establishment of an efficient cell suspension culture system for Lonicera japonica Thunb. Pak. J. Bot., 54(6): DOI: http://dx.doi.org/10.30848/PJB2022-6(7)
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