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Biosynthesis of microbial urease and its optimization for enhanced production

Yesra Arshad, Ikram Ul Haq, Muhammad Nauman Aftab and Ali Nawaz

The present study aims to produce urease enzyme from indigenously isolated microbial source. For this purpose, 55 soil samples were collected from urea-fertilized agricultural land of Punjab, Pakistan. Out of all the 42 isolated microbial strains, 35 urease positive bacteria and 7 urease positive fungal strains were screened through primary screening on Christensen medium. Secondary screening was carried out by submerged fermentation for the best urease producer. Out of all urease positive strains, S-29 strain, later identified through 16S rRNA sequencing as Lysinibacillus xylanilyticus, showed maximum urease production i.e., 1274±0.022 U/mL/min. Further, optimization of various cultural conditions was carried out for enhanced production. It was concluded that optimum cultural conditions were M5 medium (urea supplemented nutrient broth) of pH 6.5, with 24 hours of incubation at 35°C and 125 rpm, with 1.5 % inoculum size using 1 % urea as nitrogen source and 2 % glucose as carbon source. Under optimized conditions, the urease activity was increased to1335±0.182 U/mL/min

To Cite this article: Arshad, Y., I.U. Haq, M.N. Aftab and A. Nawaz. 2024. Biosynthesis of microbial urease and its optimization for enhanced production. Pak. J. Bot., 56(3): DOI:  

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