PJB-2024-119
Cloning of PgPP2C2 gene and analysis of its expression in response to nitrogen and phosphorus deficiency, and exogenous GR24 treatment in panax ginseng
Hao Liang, Hai Sun, Cai Shao, Jiaqi Qian and Yayu Zhang
Abstract
Type 2C protein phosphatases (PP2Cs) are the most abundant members of the plant phosphatase family and perform vital functions in response to various environmental stressors. To explore the role of Panax ginseng C.A. Meyer (P. ginseng) PgPP2C, a gene identified from a previously assembled transcriptome database, under adverse conditions, we created a clone of the gene and named it PgPP2C2. The length of its open reading frame was 1269 base pairs, and it coded for a 422-amino-acid protein that contained a 2C family serine/threonine phosphatase catalytic domain characteristic of the phosphatase 2C family proteins. Phylogenetic analysis of PgPP2C2 protein sequences from multiple species revealed that the P. ginseng sequence was similar to homologous sequences from Macleaya cordata, Malus domestica, Nicotiana tomentosiformis, Solanum lycopersicum, and Handroanthus impetiginosus. Transient expression analysis identified a PgPP2C2–GLosgfp fusion protein in the nucleus. The expression of PgPP2C2 was detected by transcriptional analysis in all investigated tissues, but at a higher level in the roots. Moreover, nitrogen (N) and phosphorus (P) deficiency treatments were observed to trigger PgPP2C2 expression. Specifically, the downregulation of PgPP2C2 and the accumulation of abscisic acid–glucose ester (ABA–GE) were induced under N deficiency. The synthetic strigolactone analog GR24 restored the expression of PgPP2C2 under N deficiency, but had no obvious effect on the ABA–GE content. The findings enhance our understanding of the genetic regulatory mechanism of P. ginseng in response to N and P deficiencies, as well as provide a potential strategy for improving the quality of cultivation of P. ginseng through hormone-mediated nutritional regulation
To Cite this article:
Liang, H., H. Sun, C. Shao, J. Qian and Y. Zhang. 2025. Cloning of PgPP2C2 gene and analysis of its expression in response to nitrogen and phosphorus deficiency, and exogenous GR24 treatment in panax ginseng. Pak. J. Bot., 57(4): DOI: http://dx.doi.org/10.30848/PJB2025-4(14)
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