Paper Details

PJB-2024-543

Chemical profiling and biological activities of Echeveria runyonii Rose ex. E Walter

 

Muhammad Naseer and Muhammad Adil
Abstract

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Medicinal plants are a rich source of secondary metabolites chemical compounds that can be used to develop new drugs and effectively treat serious health conditions. This investigation employed Gas Chromatography-Mass Spectrometry (GC-MS) to analyze the ethanolic and ethyl acetate extracts of Echeveria runyonii and to assess their analgesic, anti-inflammatory, and antispasmodic properties. The bioactive components in the extracts were identified by matching their mass spectra to those in the National Institute of Standards and Technology (NIST) database. Analgesic effects were evaluated using the hot plate (HP) and acetic acid-induced writhing (AAI) methods. Anti-inflammatory activity was assessed using the carrageenan-induced paw edema model (PEM), while antispasmodic effects were examined through the gastrointestinal motility test (CMT). GC-MS analysis revealed several bioactive compounds, including pharmacologically active constituents such as glycerin, cetene, trehalose, 1,2,3-propanetriol 1-acetate, pyridine-2,4-diol, 3,5,6-trimethyl-, 4H-pyran-4-one, 2,3-dihydro-3,5-dimethyl-, (E)-5-eicosene, and bis (2-ethylhexyl) phthalate. The AAI method demonstrated significant peripheral analgesic activity (p<0.001) at a 300 mg/kg dose, while the HP test showed an increase in reaction time (p<0.001) after 90 minutes. Anti-inflammatory testing revealed a reduction in paw volume by 42.86% at the same dose. The CMT indicated significant inhibition of charcoal movement, with the ethanolic extract reducing movement by 59.88% at 300 mg/kg. The identified compounds, including hexadecane and 1-octadecene, may contribute to the plant’s observed bioactivity. This study underscores the promising medicinal potential of E. runyonii, although further research is warranted to elucidate the mechanisms underlying these effects.

 

To Cite this article: Naseer, M. and M. Adil. 2026. Chemical profiling and biological activities of Echeveria runyonii Rose ex. E Walter. Pak. J. Bot., 58(1): DOI: http://dx.doi.org/10.30848/PJB2026-1(20)  
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