Paper Details
Full metadata, abstract, citation, and access status.
Breeding, growth characteristics and fungal inhibitory effect of thermotolerant Bacillus velezensis derived from distiller’s grains
Abstract
This study used a starter strain, Bacillus velezensis TS01, isolated from distiller’s grains. To directionally breed thermotolerant strains at 42-46°C for 532 h and 50 generations, EVOL (cell adaptive evolution technology) was used. Strain 46-1 has a growth of 250% higher than that of the original strain at 46°C, with stable entry into the logarithmic phase and a prolonged stationary phase. It grew optimally at 2% ethanol and 4% NaCl, grew well at pH 3, and produced amylase, protease, and cellulase. When it was tested against six pathogenic fungi, it exhibited the highest inhibition rate against Eutypella microtheca (72.60±0.99%). Then, it was followed by 58.60±4.24% and 56.53±0.11% against Mortierella gemmifera and Talaromyces fuscoviridis, respectively. 39.49±0.61% against Mortierella elongata; 38.78±0.44% against Fusarium oxysporum and 20.68±1.45% against Trichoderma harzianum. This research study provides a basis for greater biocontrol efficacy of B. velezensis in a high-temperature environment and for developing microbial fungicides
