PJB-2016-38
OPTIMIZING THE RAPID TECHNIQUE FOR PROPAGATION OF CERASUS CAMPANULATA BY TISSUE CULTURE
BIHUA CHEN1,2*, JIANMIN LI1,2, JUAN ZHANG1,2, ZHUOXI WU1,2, HUIHUA FAN1,2 AND QIANZHEN LI1,2
Abstract
The initiation medium for Cerasus campanulata was 1/2MS+1.0 mg L-1 BA+0.1 mg L-1 NAA +30 g L-1 sugar, and the average initiation rate was 94.4%. The proliferation medium composed of MS+0.3 mg L-1 BA+0.2 mg L-1 NAA+30 g L-1 sugar provided proliferation rate 3.6 and average shoot length 2.3 cm. The rooting medium composed of 1/2MS+0.6 mg L-1 IBA+20 g L-1 sugar provided rooting rate 85.6%, average root number per individual 5.9 roots, and average root length 2.7 cm. The dishwashing detergent instead of Tween-20 as the spreader reduced the contamination rate for the explant disinfection. The red-core soil adopted to be the plantlet transfer medium provided 91.3% survival rate. The light media LZ and SK were not eligible to be the transfer medium for the species.
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