PJB-2017-210
DIGITAL IMAGE ANALYSIS OF SEED SHAPE INFLUENCED BY HEAT STRESS IN DIVERSE BREAD WHEAT GERMPLASM
MUHAMMAD JAMIL, AAMIR ALI, ABDUL GHAFOOR, KHALID FAROOQ AKBAR, ABDUL AZIZ NAPAR, NAIMA HUMA NAVEED, NASIM AHMAD YASIN, ALVINA GUL AND A. MUJEEB-KAZI
Abstract
Shape changes in wheat grain under heat stress become necessary to determine when shrinkage causes quality loss. High throughput seed morpho-metery is required to quantify the shape changes precisely. In this study, shrinkage of the seed under heat stress has been quantified thus can be used as the indicator of heat stress. Randomized complete block design was followed in which 49 genotypes were examined in 16 replicates, under two treatments (normal and late sown). SmartGrain software was used to quantify the digital images of seeds picked up from two treatments for each genotype in 16 replicates. Seed shape descriptors (length, width, length width ratio, area, perimeter, circularity) were precisely and robustly determined by the use of this software. ANOVA results showed significant effect of heat stress on seed shape. Critical difference, chi-square value mentioned the disparity in seed shape of normal and stressed wheat crop. Maximum heat susceptibility index for seed area was noted (2.38) and the minimum remained (0.02). Principal component analysis (PCA) revealed the relative importance of seed shape attributes and genotype by explaining about 85.02% (F1 = 59.87 % and F 2 = 25.16 %) variability.
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