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Candidate gene association research on flavones content in hawthorn (Crataegus pinnatifida Bge.)

Zhao Yuhui, Su Kia, Zhang Liping, Li Junpeng and Guo Yinshan

In this study, 87 hawthorn (Crataegus pinnatifida Bge.) accessions were obtained from National Hawthorn Germplasm Nursery in Shenyang Agricultural University of China and flavones contents including vitexin-rhamnoside, vitexin, rutin, hyperin and quercetin were detected by high performance liquid chromatography (HPLC) in 2014 and 2015. Chalcone Synthase genes (CpCHS) of 87 hawthorn accessions were cloned and sequenced, and then SNPs (Single Nucleotide Polymorphisms) among the result of sequencing were screened out, based on thees SNPs, we conducted the association analysis between the SNPs and phenotypic traits of hawthorn flavones content, the significant associated SNPs wre then discovered. The result showed that, in total of 71SNPs were discovered and three putative causal SNPs were associated with vitexin content both in 2014 and 2015, some SNPs were associated with vitexin-rhamnoside, rutin, hyperin and quercetin. This result can be used for molecular assisted breeding of hawthorn flavone content and also provide the reference for woody plants in association analysis filed. 

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