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Abstract

Enhanced UV-B radiation has multiple effects on the morphological, physiological, biochemical, and genetic mechanisms of wheat growth. The roots of wheat seedlings treated for different numbers of days with UV-B were studied. UV-B radiation had significant effects on the number of adventitious roots, average root length, root biomass, and root vigor of wheat. Whereas root showed influence of different degree with enhanced UV-B. The best method for extracting root protein among phenyl/ammonium acetate-methanol precipitation, the TCA/acetone method, and the urea/thiourea method was determined. The TCA/acetone precipitation was found to be the best method for extracting root protein by SDS-PAGE. Finally, protein from the roots of wheat exposed to UV-B was extracted using TCA/acetone precipitation. Protein content in the UV-B radiation treatment group (B) was higher than that of the control group (CK) at the early stages of the radiation treatment; with increased time of irradiation, the protein content of B was lower than that of CK.

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