PJB-2018-426
Ultrastructure, callus induction and micropropagation of Phyla nodiflora (L.) Greene
Dalilah Abu Bakar, Rubashiny Veeramohan, Bakrudeen Ali Ahmed, Noorma Wati Haron and Rosna Mat-Taha
Abstract
The present paper reports the ultrastructure and a protocol for the efficient in vitro callus induction and micropropagation on Phyla nodiflora (L.) Greene. Foliar micromorphology study of Phyla nodiflora was investigated by JEOL-7500F Field Emission Scanning Electron Microscope (FESEM). Diacytic stomata are abundant on both the adaxial and abaxial surfaces. Trichomes are simple unicellular and densely distributed. Callus induction and shoot regenerations were achieved through culturing leaf and stem explants after disinfecting with 70% ethanol 3 minutes, 0.1% HgCl2 for 3 minutes and 20% sodium hypochlorite for 2 minutes on Murashige and Skoog (MS) medium (pH5.8) supplemented with single and combinations of auxins and cytokinins, which are naphthaleneacetic acid (NAA), 2,4-dicholorophenoxyacetic acid (2,4D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and benzyladenine (BA), kinetin (KN). Maximum 92.5% callus response on single plant growth regulators (PGRs) is 2.5 mg/L NAA (118.6 mg/L) with light green friable callus, 90.0% response from 2.0 mg/L BA (42.7 mg/L) with green compact callus and 89.2% response from combination 3.0 mg/L NAA: 2.0 mg/L KN (165.0 mg/L) with green compact and friable callus and 2.5 mg/L IBA: 1.0 mg/L BA (139.6 mg/L) with green compact callus. Highest shoot regenerations response was from MS media without plant growth regulators (90%) while shoot regenerations from MS media supplemented with combination IAA and BA gave highest multiple shoots with 3 shoots per explants. Mass propagation of callus and plantlets protocol of Phyla nodiflora can assist for future metabolic research.
To Cite this article:
Abu-Bakar, D., R. Veeramohan, B.A. Ahmed, N.W. Haron and R. Mat-Taha. 2019. Ultrastructure, callus induction and micropropagation of Phyla nodiflora (L.) Greene. Pak. J. Bot., 51(5): DOI: http://dx.doi.org/10.30848/PJB2019-5(40)
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