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Evaluation of cotton seed of advanced lines for nutritional quality through biochemical analysis

Shahwar Fatima, Sajjad Haidar and Noreen Bibi

This paper presents a study to find an advanced line of cotton which has desirable yield and quality parameters with better nutritional constituents and low amount of gossypol contents in the seed. The randomized complete block design (RCBD) with three replications was used for this experiment during 2017-18 at NIAB. Seed biochemical composition was analyzed for gossypol contents, total soluble sugars, protein fractions, amino acids, crude fibers and micro/macro nutrients. Analysis of gossypol contents revealed highly significant differences (p<0.01) among the genotypes. Gossypol content in all of advanced lines was lower than standard checks. Total amino acids were statistically highly significant among all the genotypes. For instance, the content of total free amino acids was highest (0.1686 µg/g FW) in NIAB-4-44-3 while lowest (0.0219 µg/g FW) in NIAB-4-54. There were no significant differences among advanced lines and standard checks for the contents of total soluble sugars. However, significant variations were observed for the contents of glucose and sucrose with the amount of maximum glucose in NIAB-4-54 and highest sucrose in NIAB-4-79-2. Among four protein fractions (albumin, globulin, glutelin, prolamin), albumin was abundantly present in all genotypes. Water soluble albumin fraction ranged from 3.35 mg/g fresh weight (FW) to 3.82 mg/g FW being maximum in NIAB-14-43-19 while lowest in NIAB-4-79-2. Crude fiber, crude protein, ash and other micro nutrients were also high in NIAB-4-44-3. From these results it is concluded that NIAB-4-44-3 has better nutritional quality with higher contents of amino acid and crude fiber while lowest amount of gossypol, hence, can be a better source for animal feed and human consumption

To Cite this article: Fatima, S., S. Haidar and N. Bibi. 2021. Evaluation of cotton seed of advanced lines for nutritional quality through biochemical analysis. Pak. J. Bot., 53(5): DOI:

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