Paper Details


Molecular markers and field-based screening of wheat germplasm for leaf rust resistance

Muhammad Ismail, Muhammad Rameez Khan, Aamir Iqbal, Zakir Hussain Facho, Abdullah Jalal, Iqbal Munir, Farhatullah and Sajid Ali

Leaf rust disease in wheat (caused by Puccinia triticina), is the best controlled through sustainable deployment of genetic resistance, which requires rigorous testing through field testing and marker assisted selection. A set of 28 exotic lines and three local checks were screened for leaf rust resistance using three Lr genes linked molecular markers and field testing at three locations (Lakki-Marwat, Peshawar and Mansehra). Overall leaf rust pressure was low during the wheat season of 2015-16, with maximum at Lakki-Marwat (up to 70%), followed by Peshawar (up to 50%) and minimum at Mansehra (up to 30%). The tested germplasm had variable resistance level as revealed through ACI (average co-efficient of infection); where 16 out of 28 genotypes were completely resistant, while few genotypes showed partial resistance. The maximum CI value was recorded for wheat line W-SA-87, which was 55 at Lakki Marwat, 33 at Peshawar and 15 at Mansehra, while several (18) lines had CI value of zero across the three locations. Variability existed in yield parameters with W-SA-84 (466 g per 4.5 m2 plot), W-SA-78 (443 g) and W-SA-79 (431 g) producing the better grain yield among the advance lines. Molecular genotyping revealed that STS-7 (linked with LrPr) was the most frequent (83.8%), present in 26 lines; followed by SC-Y15 (linked with Lr37) present in 24 lines (77.4%), while csLV34 (linked with Lr34) was present in 16 lines (71.1%). Interestingly, in 45% of the studied germplasm all three of the resistant genes were identified. Cluster analysis resulted in four clusters, grouping different wheat lines on the basis of both phenotypic (disease severity and yield parameters) and molecular genotypic data. These results would be useful for crossing and selection of resistance lines to reduce the leaf rust disease and ensure higher wheat yield.

To Cite this article: Ismail, M., M.R. Khan, A. Iqbal, Z.H. Facho, A. Jalal, I. Munir, Farhatullah and S. Ali. 2021. Molecular markers and field-based screening of wheat germplasm for leaf rust resistance. Pak. J. Bot., 53(5): DOI:

We Welcome Latest Research Articles In Field Of Botany The Pakistan Journal of Botany is an international journal .... Read More

Get In Touch

© 2022-23 Pakistan Journal of Botany. All Rights Reserved