PJB-2023-165
Natural neuroprotection: investigating the effects of Solanum nigrum extract on ATP-induced cell proliferation in PC12 cells
Shazia Perveen, Sumaira Kanwal, Faiza Rao, Kiran Fatima and Amina Asghar Chaudhry
Abstract
The significance of natural products in drug discovery has increased substantially, prompting intensified efforts to screen for potent drugs from natural sources. S. nigrum, commonly known as black nightshade, is documented to possess anti-proliferative, antioxidant, antimicrobial, and anti-inflammatory properties. This study aimed to investigate the impact of an extract derived from S. nigrum on ATP-induced cell proliferation in the PC12 cell line. Our findings demonstrate that exposure to S. nigrum (30 μM) led to a decrease in cell proliferation and an increase in cell death, as validated by cell counting, LDH assay, and H33258 staining. However, S. nigrum (30 µM) also significantly mitigated the harmful effects of ATP (100 μM) on the PC12 cell line. The outcomes of this research suggest that administering S. nigrum extract to the PC12 cell line for 72 hours can confer neuroprotective benefits. While the detailed impact of S. nigrum on the calcium-mediated pathway is still being analyzed, our preliminary data indicate its potential as an effective neuroprotective agent
To Cite this article:
Perveen, S., S. Kanwal, F. Rao, K. Fatima and A.A. Chaudhry. 2025. Natural neuroprotection: investigating the effects of Solanum nigrum extract on ATP-induced cell proliferation in PC12 cells. Pak. J. Bot., 57(3): DOI: http://dx.doi.org/10.30848/PJB2025-3(9)
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