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Optimize the culture and fermentation condition of biocontrol strain pH-120 against Sclerotinia sclerotiorum
Abstract
Sclerotinia sclerotiorum is the main disease in cucumber cultivation in northern China. In the early stage of laboratory, a biocontrol strain Penicillium halotolerans (Ph-120) with high-efficiency antagonistic effect on the pathogen was screened out from cucumber rhizosphere soil. In order to improve the antifungal activity of the fermentation broth of Ph-120 strain against Sclerotinia sclerotiorum, the culture conditions and fermentation conditions of Ph-120 strain were optimized by single factor test, orthogonal test and response surface methodology. The results showed that the hyphae of Ph-120 strain were affected by different single factors in the following order: temperature > nitrogen source > carbon source > pH; The optimal fermentation conditions were as follows: carbon source amount 24.0g/L, nitrogen source amount 2.2g/L, fermentation temperature 25℃, and pH value 6. The antifungal rate of the fermentation broth against pathogen was 94.6% after optimization in model verification experiment, which was 16.7% higher than that under the optimization condition. The experiment provide a theoretical basis for that development of biopesticide
