PJB-2023-457
Computational analysis of Germin-like protein genes in Brachypodium distachyon (BdGLPs)
Muhammad Ilyas, Hazrat Hussain, Muhammad Adnan Shereen, Shaheed Ullah, Muhammad Safdar Hussain, Nazish Huma Khan and Adil A. Mujawah
Abstract
Germin-like proteins (GLPs) are members of the Cupin superfamily playing an important role in plant development and inducing stress resistance. Brachypodium distachyon’s genome contains 08 GLPs which are functionally unexplored. However, in silico analysis can provide better insights into their structural, functional, and phylogenetic properties. Presently, physicochemical properties, domain composition, phosphorylation & N-glycosylation sites, subcellular localization, 3D structures, phylogenetic relationships, and functional importance of the eight BdGLPs genes were studied using the latest computational tools. All the BdGLPs exhibited similar physicochemical properties, domain architecture, 3D structures, and subcellular localizations, except BdGLP2-1, and BdGLP3-7. The Molecular and Evolutionary Genetics Analysis (MEGA11) tool was used to assess their phylogenetic relationships while STRING (Ver. 10) was used for functional characterization. All the BdGLPs showed a close phylogenetic relationship due to their presence on the same chromosome (Chr 3) except BdGLP2-1, which was found on chromosome 2. Functionally, BdGLP2-1 exhibits NADH dehydrogenase activity, whereas other BdGLPs are associated with WPP domain-related functions. This analysis provides valuable insights into the potential utilization of BdGLPs in functional genomics to develop resistant cultivars against various stresses
To Cite this article:
Ilyas, M., H. Hussain, M.A. Shereen, S. Ullah, M.S. Hussain, N.H. Khan and A.A. Mujawah. 2025. Computational analysis of Germin-like protein genes in Brachypodium distachyon (BdGLPs). Pak. J. Bot., 57(4): DOI: http://dx.doi.org/10.30848/PJB2025-4(12)
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