PJB-2024-356
Genome-wide identification and analysis of SPX gene family members in Solanum tuberosum and insights into their potential functions
Chunwei Li and Panfeng Zhao
Abstract
SPX family, named for the presence of conserved Syg1/Pho81/XPR1 (SPX) domain, is one of the most important gene families involved in phosphate ions (Pi) homeostasis and signaling transduction in plants. In this study, we identified 14 SPX family genes in Solanum tuberosum at the genome-wide level. These genes were distributed across 7 of the 12 chromosomes. Based on additional domains in the SPX family structure, the SPX family were classified into four SPX subfamilies, SPX, SPX-ERD1/XPR1/SYG1(SPX-EXS), SPX- SPX-Really Interesting New Gene (SPX-RING), and a newly identified SPX- EXS-DNL subfamily characterized by a short C-terminal motif D(N/H). The majority of SPXs were localized to the plasma membrane. Phylogenetic analysis of SPX family genes in Solanum tuberosum, Arabidopsis thaliana, Solanum lycopersicum, Oryza sativa, and Zea mays categorized these genes into 7 groups. The duplication events show that SPX genes in Solanum tuberosum were mainly produced by whole-genome duplication (WGD)/segmental duplications (SD). The synteny analysis demonstrated that the SPX gene family of Solanum tuberosum exhibited a closer phylogenetic relationship to Solanum lycopersicum compared to Arabidopsis thaliana. Additionally, expression profiling of Solanum tuberosum SPX family genes showed diverse tissue-specific patterns and varying degrees of responsiveness to both biotic and abiotic stresses. And twelve Solanum tuberosum SPX family members could interact and form an association network. Furthermore, one gene (PGSC0003DMG402022752) from the SPX subfamily was found to exhibit the highest expression levels in the floral organs, and one member (PGSC0003DMG400017163) of the SPX-EXS subfamily was highly expressed under heat treatment (35°C). These findings implies that the two genes may play a vital role in floral organ development and heat stress response, respectively. This study offers a basis for future elucidation of the functions of Solanum tuberosum SPX family genes, especially in phosphorus utilization
To Cite this article:
Li, C. and P. Zhao. 2025. Genome-wide identification and analysis of SPX gene family members in Solanum tuberosum and insights into their potential functions. Pak. J. Bot., 57(6): DOI: http://dx.doi.org/10.30848/PJB2025-6(14)
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