PJB-2024-410
Effects of ABA and nitric oxide in chickpea plants under drought stress
Fusun Yurekli and Oguz Ayhan Kirecci
Abstract
In this study, the levels of nitric oxide (NO), proline concentration, lipoxygenase (LOX) activity, malondialdehyde (MDA) level, and total chlorophyll levels were investigated in the leaves of two different chickpea varieties, sensitive (Aksu) and tolerant (Canıtez) to drought stress conditions. To achieve this aim, drought stress was induced by PEG 4000 treatment at the end of day 25. Both drought-stressed and control groups were established for both cultivars. The control group plants were irrigated with Hoagland’s culture solution throughout the experiment. Subsets of both chickpea cultivars exposed and unexposed to drought stress were treated with exogenous applications of 100 μM of sodium nitroprusside (SNP, as NO donor), cPTIO [2-(4-carboxyphenyl)-4,4,5,5 tetramethylimidazoline-1-oxyl-3-oxide potassium, as NO scavenger], and Abscisic acid (ABA) for 6 days. The treatments included Control (Hoagland’s culture solution), Control + SNP, Control + c-PTIO, Control + ABA, PEG, PEG+SNP, PEG+ c-PTIO, and PEG+ ABA groups. Leaf samples were collected on days 0, 3, and 6 for analysis. All experiments, including germination and growth stages, were conducted under controlled conditions in a plant growth chamber. According to the results obtained, inhibition of nitric oxide synthesis led to increased oxidative stress. Similarly, application of abscisic acid alleviated the adverse effects of drought. Furthermore, prolonged drought stress conditions resulted in more oxidative damage, and both nitric oxide and abscisic acid exhibited positive effects in this scenario as well
To Cite this article:
Yurekli, F., and O.A. Kirecci. 2025. Effects of ABA and nitric oxide in chickpea plants under drought stress. Pak. J. Bot., 57(6): DOI: http://dx.doi.org/10.30848/PJB2025-6(22)
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