Paper Details

PJB-2024-425

Phylogenetic analysis of some endemic mosses of northern Pakistan

Imran Ahmad, Waheed Murad and Abdul Nasir Khalid
Abstract

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The study has been focused on morpho-anatomical and molecular characterization of some taxa from the families Fissidentaceae and Grimmiaceae in district Dir Upper, Khyber Pakhtunkhwa Pakistan. A sequence and the phylogeny of Fissidens dubius, Fissidens grandifrons, and Grimmia ovalis was performed for the first time from Pakistan. Nuclear Internal transcribed spacer (ITS1) region was used to find out the phylogenetic position of these taxa. Phylogenetic analyses sequences of the nrDNA ITS1 (BMBC-LS4) region of collections (IM25, IM27, IM54 and IM31) were obtained with both the primers. Evolutionary history was inferred by using the software Maximum Likelihood method based on the Tamura-Nei model. Fissidens dubius and Fissidens grandifrons are described as new report from the study area. Our findings describe the potential of ITS1 in identification and classification of the species. Description of these species along with local photographs and phylogenetic tree are provided. The current attempt is the first one of its natures to describe the molecular characterization of mosses from district Dir upper. It will help the researchers for further taxonomic studies in exploring moss flora of the study region and will lead a way forward for more research in ecological and phytochemical fields.

To Cite this article: Ahmad, I., W. Murad and A.N. Khalid. 2026. Phylogenetic analysis of some endemic mosses of northern Pakistan. Pak. J. Bot., 58(1): DOI: http://dx.doi.org/10.30848/PJB2026-1(26)  
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