PJB-2024-499
Revealing the potential of Azadirachta indica A. Juss. for the induction of amylolytic and pullulytic enzymes
Tuba Nafees, Shafaq Ayaz Hassan and Saifullah
Abstract
In vitro culture of Azadirachta indica offers controlled and constant provision of metabolites and therefore could be more feasible in the enzyme induction study. In this research, we developed cell suspension cultures through neem callogenesis and incorporated different percentages of inducers, namely starch, dextrin, and pullulan in the suspension media to analyze their effects on the induction of amylolytic and pullulytic enzymes. The cell-free extracts of the induced cultures were analyzed on starch and pullulan agar-solidified plates for the zones of hydrolysis. Additionally, enzyme activities were quantified using the DNS method. Consequently, starch and pullulan degrading activities were detected in the cultured media, confirming the successful induction of amylolytic and pullulytic enzymes. These enzymes hold significant commercial value in the food, textile, and detergent industries. This research represents the first report in the literature on pullulan degradation by neem enzymes and offers valuable insights for future research in this area
To Cite this article:
Nafees, T., S.A. Hassan and Saifullah. 2025. Revealing the potential of Azadirachta indica A. Juss. for the induction of amylolytic and pullulytic enzymes. Pak. J. Bot., 57(6): DOI: http://dx.doi.org/10.30848/PJB2025-6(41)
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