Paper Details

PJB-2025-11

Utilization of novel EST-SSR markers to explore the genetic diversity and population structure of Polygonum cuspidatum

Guohui Shi, Rukhsar Shaheen, Mohammad Murtaza Alami, Fengfeng Li, La Ji, Xueting Zhao, Yonghong Zhang, Lanlan Zheng, Beibei Luo, Xuekui Wang, Duanyang Weng, Shumei Zhong, Shengqiu Feng and Shaohua Shu
Abstract

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Polygonum cuspidatum, a medicinal plant renowned for its therapeutic properties, also poses ecological challenges as an invasive species in Asia and Europe. Despite extensive research on its pharmacological applications and management strategies, its genetic diversity is inadequately comprehended. This study involved an analysis of 52 wild individuals collected from four provinces in China (Hubei, Hunan, Guizhou, and Sichuan) to fill this knowledge gap. By utilizing 24 novel expressed sequence tag-simple sequence repeat (EST-SSR) markers, we determined 191 highly polymorphic alleles, revealing a rich and complex genetic landscape that underscores the potential for conservation and breeding efforts. This study provides an assessment of genetic variation using newly developed EST-SSR markers. It also demonstrates a foundation for sustainable utilization and genetic improvement of P. cuspidatum.

 

 

To Cite this article: Shi, G., R. Shaheen, M.M. Alami, F. Li, L. Ji, X. Zhao, Y. Zhang, L. Zheng, B. Luo, X. Wang, D. Weng, S. Zhong, S. Feng and S. Shu. 2026. Utilization of novel EST-SSR markers to explore the genetic diversity and population structure of Polygonum cuspidatum. Pak. J. Bot., 58(1): DOI: http://dx.doi.org/10.30848/PJB2026-1(24)  
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