Paper Details
Full metadata, abstract, citation, and access status.
Identification and validation of reliable reference genes in Saposhnikovia divaricata under low-temperature stress
Abstract
Based on transcriptomic data, this study screened and validated reference genes in Saposhnikovia divaricate under low-temperature stress. Quantitative real-time polymerase chain reaction (qRT-PCR) experiments were conducted, combined with computer software tools such as geNorm, NormFinder, Best Keeper, Delta Ct, and Ref Finder, to identify stably expressed reference genes in the leaves, stems, and roots of S. divaricata. The reliability of the selected reference genes was verified by analyzing the expression changes of the sucrose phosphate synthase (SPS) gene under low-temperature conditions. A total of 8 candidate reference genes were screened in this study. The results showed that 18SrRNA-2 exhibited the most stable expression in roots and stems, while 18SrRNA-1 showed the most stable expression in leaves. These findings are of great significance for subsequent studies on changes in the content of active substances in S. divaricate under abiotic stress, as they can improve the standardization of qRT-PCR experiments and provide support for research related to gene expression in S. divaricata.
