PJB-2008-194
EXPLOITATION OF POTENTIAL TARGET TISSUES TO DEVELOP POLYPLOIDS IN CITRUS
MUHAMMAD USMAN1 , BILQUEES FATIMA1, KAZIM ALI GILLANI1, MUHAMMAD SARWAR KHAN2, MUHAMMAD MUMTAZ KHAN1
Abstract
One of the rich sources of germplasm development to improve citrus crop is the ploidy management through in vivo and in vitro techniques. In vitro and in vivo applications of colchicine change the chromosome number by interfering with chromosomal segregation at anaphase, restricting cell wall formation and yielding polyploid cells. Further, endosperm culture under aseptic conditions also allows regeneration of plants with variable genetic make up. We report here the development of polyploids through in vitro culturing of endosperm and application of colchicine. Somatic embryos were developed from ‘Kinnow’ mandarin (Citrus reticulata L.) and ‘Succari’ sweet orange (Citrus sinensis L. Osbeck) on basal medium modified with higher concentrations of BA and NAA (10mgL-1 and 2mgL-1, respectively) from calli induced on Murashige and Tucker (MT) medium supplemented with BA
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