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IN VITRO REGENERATION POTENTIALITY OF OIL SEED BRASSICA GENOTYPES WITH DIFFERENTIAL BAP CONCENTRATION
Abstract
Petiole of six genotypes of oilseed Brassica viz., Tori-7, Sampad, Kallyania, BARI Sarisha-7, BARI Sarisha-8 and MM 20-3 were cultured in MS medium with different concentrations of BAP, NAA and AgNO3 for callus induction and subsequent plant regeneration. The highest percentage of callus induction (91.43%) was observed in Tori-7 with the media supplemented with 2 mgL-1BAP, 0.1 mgL-1 NAA and 2.0 mgL-1 AgNO3. Calli were maintained in order to get sufficient number of regenerants. With the increased concentration of BAP, the highest percentage (57.14) of regenerants were found in Tori-7 followed by Sampad (33.13%) and BARI Sarisha-8 (31.42%) in MS medium supplemented with 2.5 mgL-1 BAP, 0.1mgL-1 NAA and 2.0mgL-1 AgNO3. Root formation from the regenerants was found best in half MS medium supplemented with 0.5mgL-1 NAA in genotype Tori-7. Regenerated plantlets of 4 genotypes (Tori-7, BARI Sarisha-8, Kallyania, BARI Sarisha-7) were successfully established in the field.
