PJB-2018-1809
The Cyanobacterium hemoglobin (CHb) promotes the growth of bacteria, yeast and Brassica napus, and enhances submergence resistance of Brassica napus
Zhu Keming, Li Kaixia, Li Juan, Xu Shuo, Sundus Zafar and Tan Xiaoli
Abstract
The coding sequence of Cyanobacterium hemoglobin (CHb) gene, from Cyanobacterium Synechocystis sp. PCC 6803, was inserted into vectors pET-30a, pYES2 and pCAMBIA1300. The expression of CHb in E. coli (BL21) and yeast (pep4) could enhance the growth rate of bacteria and yeast. Constitutive CHb over-expression in Brassica napus induced earlier germination of transgenic seeds and increased chlorophyll content in transgenic seedlings. Under submergence conditions, the bioassays revealed that CHb over-expression protected transgenic plants against prolonged waterlogging. These results suggested that CHb gene could promote the growth rate of microbes and Brassica napus, and resistance to submergence for Brassica napus.
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