PJB-2023-116
Green synthesis of silver nanoparticles using wild mushroom Lepista sordida and its antifungal activity
Tayyaba Khalil, Samina Sarwar, Sumera Javad, Aqsa Abid, Muhammad Hanif, Hajra Khalil and Amina Abrar
Abstract
Macrofungal-assisted synthesis of nanoparticles has been reported to produce a large amount of protein with high yield and low toxicity in many countries. However, it is a neglected filed in Pakistan. The aim of this research was to investigate the potential of wild neglected mushroom Lepista sordida occurring in Pakistan to synthesize silver nanoparticles (AgNPs) through economical and eco-friendly methods and to evaluate their antifungal potential against pathogenic fungi. This mushroom was examined first time for synthesis of its nanoparticles in Pakistan. L. sordida was also first time analyzed from Pakistan through molecular analysis. The synthesis of AgNPs (through Microwave assisted extraction method) was confirmed and characterized by ultraviolet (UV)- visible spectroscopy, Scanning electron microscopy (SEM), and X-ray diffraction analysis. UV-visible spectrum for silver nanoparticles exhibited a broad absorption peak between 200–800 nm and showed a maximum absorption spectrum at 345 nm, which indicated the existence of AgNPs. SEM micrographic studies confirmed that synthesized AgNPs from L. sordida extract had smooth hexagonal morphology in the nano-range. The antifungal activity was performed against the pathogenic micromycetes species (Aspergillus flavus and Alternaria alternata) to check the maximum inhibitory effect of AgNPs against these species. The AgNPs synthesized with fungus extract inhibited the growth of A. flavus and A. alternata by 97.3% and 91.75% at the concentration of 10µg/L. This indicated that the synthesized AgNPs using fungus had the capability to control pathogen's growth with low toxic rate and good biocompatibility mode. This work can be helpful in future projects related to the development of nanofungicides against fungal pathogens
To Cite this article:
Khalil, T., S. Sarwar, S. Javad, A. Abid, M. Hanif, H. Khalil and A. Abrar. 2025. Green synthesis of silver nanoparticles using wild mushroom Lepista sordida and its antifungal activity. Pak. J. Bot., 57(3): DOI: http://dx.doi.org/10.30848/PJB2025-3(11)
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