PJB-2023-566
Transcriptome analysis of Pinellia ternata during leaf development
Hongyu Chen, Chunbo Liang, Yantao Liang, Longyan Tan, Ying Yu and Weizhong Li
Abstract
Pinellia ternata (Thunb.) Breit., a widely distributed medicinal plant in China, is highly valued for its alkaloids, guanosine, organic acids, and other bioactive compounds. While various transcription factor (TF) families have been implicated in P. ternata growth, development, and abiotic stress responses, the absence of a complete P. ternata genomic sequence has hindered deeper understanding of the precise molecular regulatory mechanisms governing P. ternata gene expression during leaf growth. In this study, we performed transcriptome and gene expression profiling of leaves across four developmental stages to unveil specific gene expression patterns for each stage. Sequencing of the leaf transcriptome encompassing these stages generated over 45.44 million raw reads, yielding approximately 42.04 million clean reads per sample. De novo assembly of high-quality reads generated 221,251 unigenes (UniGs) averaging 773 bp in length. Analysis of UniG sequences revealed dynamic transcriptomic changes occurring during P. ternata growth and development, including significant changes in expression of genes related to several TF families. Subsequent exploration of the annotated RNA-seq database uncovered numerous TFs exhibiting high-level, stable expression during specific leaf developmental stages, suggesting pivotal roles for some TFs in P. ternata leaf growth and development. This comprehensive analysis provides insights into the intricate regulatory networks underlying P. ternata leaf development, paving the way for future functional genomics studies and targeted improvement of this valuable medicinal plant
To Cite this article:
Chen, H., C. Liang, Y. Liang, L. Tan, Y. Yu and W. Li. 2025. Transcriptome analysis of Pinellia ternata during leaf development. Pak. J. Bot., 57(3): DOI: http://dx.doi.org/10.30848/PJB2025-3(18)
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