PJB-2024-37
Foliar applied 5-aminolevulinic acid ameliorated the adverse effects of heavy metals (Cd and Pb) by triggering antioxidant system in two varieties of mustard (Brassica campestris L.)
Huma Riaz, Khalid Nawaz, Khalid Hussain, Noshia Arshad, Iqra Iqbal, Noor Fatima, Mehreen Javeria, Samina Zafar and Taswar Ahsan
Abstract
Heavy metal (HM) stress has adverse effects on growth of plant and biochemical parameters. The foliar spray of plant growth regulators is a renowned approach to alleviate the negative effects of HM on plants. It is considered that the influence of HM on plant physiological phenomenon can be overcome by the use of amino acids. Therefore, the major objective of present study was to find the role of 5-aminolevulinic acid (ALA) in alleviating the Heavy metals stress in two varieties i.e. Super canola-2018 (V1) & Super Raya-2016 (V2) of mustard. A pot experiment was conducted in Botanical Garden, Department of Botany, University of Gujrat, Gujrat, Pakistan. Total ten treatments were used and each treatment was replicated four times. A Completely Randomized Design (CRD) was used for this experiment. Pb (100µM) and Cd (100µM) were applied in the form of PbCl2 and CdCl2 at the time of sowing to induce heavy metal stress. After two weeks of germination, 5-ALA (50mg/L& 100mg/L) was foliarlly applied. Results showed that HMs stress reduced germination by 44%, fresh & dry biomass of root 77% & 73% and fresh & dry biomass of shoot 80% & 79% respectively. Similar trend was also observed for length of shoot (46%) and root (71%). Biochemical parameters like carbohydrates and proteins were reduced by 29% & 33% respectively. However, the foliar spray of 5-ALA (100 mg/L) improved morphological, biochemical and physiological parameters under HMs stress. Biochemical parameters including chl-a 41%, chl-b 13% & carotenoids 27% soluble protein contents by 34% were increased under stressed environment. Antioxidant enzymes activities was higher under HMs stress which was decreased (CAT 76%, POD 40%, SOD 38%) by foliar application of 5-ALA. Adverse effects of HMs were more severe in variety Super canola-2018 than variety Super Raya-2016. It was concluded that 5- Aminolevulinic Acid could be useful to reduce the effect of HM from mustard
To Cite this article:
Riaz, H., K. Nawaz, K. Hussain, N. Arshad, I. Iqbal, N. Fatima, M. Javeria, S. Zafar and T. Ahsan. 2025. Foliar applied 5-aminolevulinic acid ameliorated the adverse effects of heavy metals (Cd and Pb) by triggering antioxidant system in two varieties of mustard (Brassica campestris L.). Pak. J. Bot., 57(3): DOI: http://dx.doi.org/10.30848/PJB2025-3(29)
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