PJB-2012-242
EFFECT OF GAMMA IRRADIATION ON ASPERGILLUS NIGER FOR ENHANCED PRODUCTION OF GLUCOSE OXIDASE
MUHAMMAD ANJUM ZIA1,*, SAMREEN RASUL1 AND TEHREEMA IFTIKHAR2,3
Abstract
Developing countries have a high prevalence of diabetes and their populations are suffering from associated adverse factors. Such a frequency requires more effective diagnosis, mostly achieved by glucose diagnostic kits. Although high priced kits are available in market but local production of such kits can be highly cost effective and may confer the decline in incidence of the disease. Glucose oxidase is the key enzyme for the determination of glucose in such analytical tools. Enhanced production of glucose oxidase was performed by mutagenesis of Aspergillus niger by gamma irradiation. A dose of 80krad was found as optimum for derivation of positive mutant strains. Following the screening by triton X-100 and 2-deoxy-D-glucose, the selected strains A. niger G-80-A, A. niger G-80-B and A. niger G-80-C showed 27.5
To Cite this article:
Download
